Coding

Part:BBa_K3796004:Design

Designed by: Li Mingyang   Group: iGEM21_CAU_China   (2021-09-04)


capC from Bacillus subtilis str.168


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 81
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A segment of the RBS recognized by the ribosome of Corynebacterium glutamicum and a promoter recognized by the sigma factor needs to be attached upstream of this sequence, as well as a segment of the terminator downstream.


Source

Bacillus subtilis str.168 genome.

References