Coding
Part:BBa_K3796004:Design
Designed by: Li Mingyang Group: iGEM21_CAU_China (2021-09-04)
capC from Bacillus subtilis str.168
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 81
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A segment of the RBS recognized by the ribosome of Corynebacterium glutamicum and a promoter recognized by the sigma factor needs to be attached upstream of this sequence, as well as a segment of the terminator downstream.
Source
Bacillus subtilis str.168 genome.